Estudo comparativo entre as técnicas de Imunofluorescência Direta, Imunofluorescência Indireta e Coloração de Sellers em amostra de tecido nervoso de cães e de animais silvestres para o diagnóstico da cinomose

Abstract

It was performed a study comparative in the diagnostic of the canine distemper through techniques of Direct Immunofluorescence (IFD), Indirect Immunofluorescence (IFI) and Stain Sellers in samples of nervous tissue of dogs and wild animals that died with suspicion clinical of the viruse. Were utilized 118 samples of central nervous system (SNC) of domestic dogs and 3 wild animals, 2 skunks and 1 monkey, utilizing Ammon´s horn (CA), cerebellum (CB) and brain stem (TE). The TE was the region of larger percentage of positive, following of CA. The comparation between Stain Sellers and IFD, this last demonstrated a large sensitive. The IFI observed what to the realization of the technique was necessary the utilization of fresh materials. The utilization of the 117 samples in conditions of autolysis presented negative result. However 1 sample not autolysate, it was result positive in the 3 brain regions (CA, CB, TE). It was not possible the comparative with the technique of IFI, because in the work the technique alone was possible in materials conserved, with the advantage of a read more easy. In the work was detected also canine distemper in a skunk through technique of IFD.