Isolamento de Cryptococcus neoformans (SANFELICE) VUILLEMIN (1901) de Periplaneta americana (LINNEU, 1758) recolhidas de ambientes públicos

Abstract

The objective of this paper was determined the presence of Cryptococcus neoformans in cockroach (Periplaneta americana) collected in public places. The motivation for this research was based on the fact that this species of cockroach is synanthropic and vehicle of several pathogenic agents for man and animals. The insect were collected already dead and also collected alive using different traps, at all, were 150 samples. The research of the presence of the agents in the insects was made in Laboratory of Pathogenic and Environmental Yeasts, Department of Microbiology and Immunology Veterinary, Institute of Veterinary, UFRRJ, by the process of the exoskeleton and the internal content of then, sowing in cultivation medium containing dopamine. The confirmations of isolation were made by macromorfology and micromorfology test, realization of auxanographic method and complementally proofs. Was not obtained isolation of Cryptococcus neoformans of exoskeleton. Were obtained 5 isolated (3%), starting by the content of the insects that, after the serogrouping in CGB medium, show them self as belonging to AD serum group. Test show that 80% of the stumps are big producing of phospholipase and 100% big producing of protease. We considerate that the cockroaches of the species Periplaneta americana are,potentially, a vector for the studied yeast.