Levantamento da fauna flebotomínica e ocorrência de cães sororreagentes para Leishmaniose Tegumentar Americana no Município de Seropédica, Estado do Rio de Janeiro

Abstract

The American Cutaneous Leishmaniasis (ACL) is a no contagious infectious illness caused by Leishmania protozoa genera affecting skin and mucous membranes basically being a zoonotic infection. The aim of this study was to cany out phlebotominic fauna survey in ACL human cases occurrence areas. For sandfly collection monthly systematic captures by Castro trap from 6 o clock p.m. to 10 o clock p.m. for 12 months were performed. The diptera were placed on 70% alcohol bottles and carried to Sergio Arouca Public Health National School Biological Sciences Department Vectors Laboratory, Oswaldo Cruz Foundation, Rio de Janeiro, where they have been clarified and identified, as well. For dogs serology, blood samples from invaded residences animals as well as from a neighbour one, by venous puncture were taken. After serum centrifugation and separation, samples were stored at 20ºC till serological assays performance at FIOCRUZ Public Health Nation School Immunodiagnosis Laboratory. The following methods: Indirect Immunofluorescence Reaction (IFIR) and Enzyme-linked immunosorbent assay (ELISA) were used. From October, 2004 to September, 2005, 2390 units from four species were captured. The predominant species was the Lutzomyia intermedia with 97.1% from total collected, followed by L. whitmani 1.6%, L.migonei 1.21% and L.oswaldoi 0.09 %. From 35 dogs serology: 60% (21) no reagent animals were reported, 22.9% just for ELISA, 2.9% for IFIR, and 14.2% (5) reagent animals for both techniques, definitely positive. No correlated evidences between serologies (IFIR+/ELISA+ and other results) in regard to animals residence (ill or neighbour) by Fisher statistical test were reported. It might be suggested that ACL transmission in Seropédica County has basically been spread by Lutzomyia intermedia specie. The presence of reagent dogs unless suggestive injury may contribute for new ACL reports.