Avaliação experimental e estudos a campo relacionados a Theileria equi (Laveran, 1901) Mehlhorn & Schein, 1998 em Canis familiaris no Município de Seropédica - Estado do Rio de Janeiro, Brasil

Abstract

The equines piroplasmosis are diseases that may cause debilitating progress or fatal episodies, its etiologic agents are the species Theileria equi and Babesia caballi. The maintenance of these species in nature, as well as the widespread ability, are limitants factor to genetic improvement of equines herds in view of the internationals restrictions referring the accomited animals transit with these patogens. In Brazil, in economic terms these patogens have a great importance because the national erd is stimated in 5,800,000 of animals, that dropped with the asinines, arrive in 8,300,000 of animals. The eluciding of links to the natural disease history, overall the ones that propitiate the perpetuation of it in our herds, becomes urgent and necessary. Note that some species have a sight convivence with equines, among it is the Canis familiaris. With purpose to evaluate the possibility of C. familiaris to be involved on epizootiologic natural chain of T. equi, we used two boarding: experimental and epizootiologic. For the first, using material from seventeen dogs and puppies that were divided in three groups: G1 Puppies; G2 Dogs; G3 immunossuprimitted dogs and puppies, both ixodids-free since birth until end of experimental evaluations referring the possibility to C. Familiaris to be a able host to T. equi. to evaluate the occurrence of T. equi in these animals, were used some diagnostic tools, as follow: parasitologic aspect blood smears in immersion optical microscopy (IOM); sorology: using research of antibodies against T. equi, with immunofluorescence indirect test (IFT); and molecular using four differents protocols of polimerase chain reaction (PCR), in follow material: blood with EDTA; fragment of tissues; Boophilus microplus nymphs and Rhipicephalus sanguineus in adult stage. In the second moment, evaluated, for three times, twelve dogs from enzootic stability area to T. equi with a sight convivence with horses since birth occasions, using for it IMO and IFT, forming G4 group. The results were negatives in all observations. Concluding that the T. equi specie did not show the ability to establishment and development in C. Familiaris; and that specie do not involved in epizootiologic natural chain of T. equi, in municipality of Seropedica, State of Rio de Janeiro, Brazil.