Modulação do processo de lignificação por aplicação de ácido jasmônico e ácido 2,4-diclorofenoxiacético em Eucalyptus urophylla S. T. Blake

Abstract

Lignin is a important a biomacromolecule for several biologic process in plants, the most important process is to give necessary rigidity to keep the stem verticality, and water and minerals circulation. This research was carried out with Eucalyptus urophylla seedling and the result can help other biothecnological studies. In this research were applied different growth regulators concentrations such as jasmonic acid (1μM e 2 μM) and 2,4-diclorofenoxiacético (18,12 μM e 36,24 μM). Analysis of the treated plants allowed to understand some unknown questions on biochemical and anatomical point of view. The experimentwas factorial with whole ramdomized desing. It was get insoluble (Klason) and dioxane lignins. Infrared spectroscopy, total protein, methoxyl determination and anatomical study were carried out. The results showed that the jasmonic acid on its first concentration activated the lignin formation process, while its second this process was disabled. 2,4-D (A1) and A2 treatments contributed for the lignification process, however with the second provid less reduction to be comper with stander treatment. The conjugated treatments J1A1 e J1A2 show to have effect on lignification with high sinergetic behaviour for jasmonic acid, activing the lignin formation. The result waved for lignin modification process, as well as the alteration in the protein and anatomy.