“Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.

Abstract

There are few industrial duck farms in Brazil, because for the national population, the meat and eggs of this bird are still considered restricted. Subsistence creations are common in the country, and trade in live, slaughtered poultry, eggs and meat mainly occurs among small producers, commercial houses and free markets. There are no records or knowledge of the hygienic sanitary conditions where these birds are created. The class of birds is susceptible to infection by three species of Cryptosporidium (Cryptosporidium meleagridis, C. baileyi and C. galli), in addition to these 13 genotypes. The objective of this study was to diagnose the presence of Cryptosporidium spp. in the domestic duck of the family Anatidae (Cairina moschata), from two rustic creations located in Rio de Janeiro; Carry out the genotypic characterization followed by sequencing and phylogenetic analyzes, comparing with the sequences obtained with those sequences deposited in GenBank. As a methodology, fecal samples of domestic ducks (Cairina moschata) were collected in two rustic creations (Area A and B), totaling 118 samples, 60 samples from area A and 58 from area B. The animals of the collection were young birds that did not exceed 30 days of life. Initially the research of oocysts Cryptosporidium spp. was carried out, through the technique of centrifugation and flotation in saturated solution of sugar. In the microscopic diagnosis, 20 samples from area A showed positivity (118 / 16.95%). Of these, we selected those that had more than 20 oocysts of Cryptosporidium spp. By microscopic field for the extraction of DNA, followed by molecular diagnostic methodologies targeting 18S, which were later, sequenced and phylogenetic analyzes were performed. In this study, the species Cryptosporidium baileyi, was diagnosed, being more closely related to the gastric species, being a species of importance in animal health