Isolamento e estudo da interação da mangiferina e derivados sintéticos com albumina sérica humana

Abstract

Mangiferin is a polyphenolic compound naturally isolated from leaf extracts of the mango tree (Mangifera indica), with mango being one of the most appreciated fruits in the world. In addition to the economic importance of M. indica, in terms of the commercialization of its fruits and products, mangiferin has different pharmacological activities, especially antioxidant, anti-inflammatory, immunomodulatory, antitumor, antiviral, anthelmintic and antibacterial activity. Its structural characteristics, together with the abundance of mangiferin in the vegetal material and the ease of its extraction, qualify it as an extremely attractive natural product for evaluation in different biological models, besides making possible its use as starting material, aiming the planning of Derivatives with potential pharmacological applications. In this work the mangiferin, a glycosylated xanthone, was the major compound obtained after the extraction of the leaves of Mangifera indica, by Soxhlet and microwave, characterized by its melting point, infrared spectra and 1 H and 13 C NMR. The quantification of mangiferin in the extracts and their purity of 99.9% was determined by high performance liquid chromatography with a diode arrangement detector. The in vitro antioxidant capacity of the extract was performed by the radical capture method (DPPH). The structural modification of mangiferin occurred through the reaction of acetylation, selective acetylation, alkylation and benzylation. The synthesized products were suitably characterized by melting point, NMR spectrometry, infrared, HPLC-DAD and high resolution mass spectrometry. The interaction between mangiferin (MGF) and its derivatives with human serum albumin solution was investigated by spectroscopic (fluorescence and circular dichroism) and computational (molecular docking) studies. The compounds were evaluated for their cytotoxic capacity in vitro against the human monocyte line THP-1.