Modificação da técnica de Whitlock (1959), para o diagnóstico de nematóides gastrintestinais de bovinos

Abstract

With the objective of lessening the time between the sampling and diagnosis of infections due to gastrointestinal nematodes in bovines, modifications were made of the WHITLOCK (1959) technique. Basic changes consisted of the exclusion of the negative pressure pump, used in removing the liquid during the collection of eggs; the exclusion of the Buchner filter in the filtration of the suspension and the substitution of the bottle used by WHITLOCK (1959) by one adapted by the author. These modifications apart from reducing costs, gave good results under routine laboratory conditions due to increased simplicity. First stage larvae (L1) thus obtained may be identified in a shorter period (24 hours) than that needed for infective larvae (7 days). Pure cultures were prepared and these larvae were considered as "reference larvae" for the identification of 43 mixed cultures derived from eggs in faeces. In this study, observations were made on Haemonchus contortus (ovine), Haemonchus placei, Cooperia spp., Trichostrongylus spp, and Oesophagostomum radiatum (bovine), measurements and descriptions of their morphological characters are given.