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dc.creatorRodrigues, Maria de Lurdes de Azevedo-
dc.creator.Latteshttp://lattes.cnpq.br/1288032325091121por
dc.contributor.advisor1Honer, Michael Robin-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/5039574335668169por
dc.date.accessioned2020-10-15T12:41:20Z-
dc.date.issued1980-01-02-
dc.identifier.citationRODRIGUES, Maria de Lurdes de Azevedo. Modificação da técnica de Whitlock (1959), para o diagnóstico de nematóides gastrintestinais de bovinos. 1980. 70 f. Dissertação (Mestrado em Ciências Veterinárias) - Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica-RJ, 1980.por
dc.identifier.urihttps://tede.ufrrj.br/jspui/handle/jspui/3982-
dc.description.resumoObjetivando-se diminuir o tempo entre amostragem e o diagnóstico das infecções por nematódeos gastrintestinais de bovinos, modificou-se a técnica de WHITLOCK (1959). A mudança básica consistiu na não utilização da bomba de pressão negativa para retirada do líquido na obtenção de ovos e do filtro de Buchner para a filtração da suspensão e na substituição do frasco usado por WHITLOCK (1959) por outro adaptado por nós. Essas modificações tornaram o processo menos oneroso e facilitaram seu uso na rotina laboratorial dando bons resultados devido à sua fácil execução. As larvas de primeiro estágio (L1), assim obtidas, podem ser identificadas em um período de tempo menor (24 horas) do que o utilizado para a larva infectante (7 dias). Preparam-se culturas puras e as larvas, assim obtidas, foram consideradas como larvas de referência para comprovar a identificação das larvas de culturas mistas provenientes dos ovos coletados nas fezes. 41 No presente estudo trabalhou-se com Haemonchus contortus de ovinos, Haemonchus placei, Cooperia spp., Trichostrongylus spp. e Oesophagostomum radiatum de bovinos, apresentando-se medidas e descrição das características, morfológicas.por
dc.description.abstractWith the objective of lessening the time between the sampling and diagnosis of infections due to gastrointestinal nematodes in bovines, modifications were made of the WHITLOCK (1959) technique. Basic changes consisted of the exclusion of the negative pressure pump, used in removing the liquid during the collection of eggs; the exclusion of the Buchner filter in the filtration of the suspension and the substitution of the bottle used by WHITLOCK (1959) by one adapted by the author. These modifications apart from reducing costs, gave good results under routine laboratory conditions due to increased simplicity. First stage larvae (L1) thus obtained may be identified in a shorter period (24 hours) than that needed for infective larvae (7 days). Pure cultures were prepared and these larvae were considered as "reference larvae" for the identification of 43 mixed cultures derived from eggs in faeces. In this study, observations were made on Haemonchus contortus (ovine), Haemonchus placei, Cooperia spp., Trichostrongylus spp, and Oesophagostomum radiatum (bovine), measurements and descriptions of their morphological characters are given.eng
dc.description.provenanceSubmitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2020-10-15T12:41:20Z No. of bitstreams: 1 1980 - Maria de Lurdes de Azevedo Rodrigues.pdf: 502275 bytes, checksum: 35f1806adfeef214857af3371ac4858d (MD5)eng
dc.description.provenanceMade available in DSpace on 2020-10-15T12:41:20Z (GMT). No. of bitstreams: 1 1980 - Maria de Lurdes de Azevedo Rodrigues.pdf: 502275 bytes, checksum: 35f1806adfeef214857af3371ac4858d (MD5) Previous issue date: 1980-01-02eng
dc.description.sponsorshipCoordenação e Aperfeiçoamento de Pessoal de Nível Superior - CAPESpor
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico - CNPqpor
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dc.languageporpor
dc.publisherUniversidade Federal Rural do Rio de Janeiropor
dc.publisher.departmentInstituto de Ciências Biológicas e da Saúdepor
dc.publisher.countryBrasilpor
dc.publisher.initialsUFRRJpor
dc.publisher.programPrograma de Pós-Graduação em Ciências Veterináriaspor
dc.relation.referencesAHLUWALIA, J.S. & CHARLESTON, W.A.G., 1974. Studies on the de velopment of the free-living stages of Cooperia curticei. New Zealand Ver. J., 22(10):191-195. ANDREWS, J.S., 1939. Life history of the nematode Cooperia curticei, and development of resistance in sheep. J. Agric. Res., 58(10) :771-785. BIRD, A.F., 1971. The structure of nematodes. Academic Press. N.Y., U.S.A., 318p. CHITWOOD, L.M. & CHITWOOD, M.B., 1974. Introduction to nematology. University Park Press, Baltimore, 364p. CROFTON, H.D. & WHITLOCK, J.H. ,1965. Cap.12 In: BIRD,A.F., 1971. The structure of nematodes. Academic Press, N.Y.,279-300p. CUNLIFFE, G. & CROFTON, H.D., 1954. Egg size and differential egg counts in relation to sheep nematodes.Parasitology, 45:275-286. 45 DICKMANS, G. & ANDREWS, J.S., 1933. A comparative morphological study of the infective larvae of the common nematodes parasitic in the alimentary tract of sheep. Trans. Am. Micr. Soc., 52:1-25. DOUVRES, F.W., 1957. The morphogenesis of the parasitic stages of Trichostrongylus axei and T. colubriformis, nematode parasites of cattle. Proc. H£1m. Sic. Wash.,24(1): 4-14. FARR, M.M. & LUTTERMOSER, G.W., 1941. Comparative efficiency of zinc sulfate and sugar solutions for the simultaneous flotation of coccidial oocysts and helmintic eggs. J. Parasitol., 27:417-424. GIBBONS, L,M., 1979. Revision of the genus Haemonchus Coob, 1898 (Nematoda: Trichostrongylidae). Systematic Parasitology, 1 (1) :3-24. GOLDBERG, A., 1951. Life history of Oesophagostomum venolosum, a nematode parasite of sheep and goats. Proc. Helm. Soc. Wash., 18(1):36-47. GORDON, H. McL., 1967. The diagnosis of helminthosis in sheep. Vet. Med. Review, 2/5:140-168. GORDON, H. McL & WHITLOCK, H.V., 1939. A new technique for counting nematode eggs in sheep faeces. J.Council Scient. Indust. Res. Australia, 12:50-52. 46 HANSEN, M.F. & SHIVNANI, G.A:, 1956. Comparative morphology of infective nematode larvae of Kansas beef cattle and its use in estimating incidence of nematodiasis in cattle. Trans, Am. Mi cr. Soe., 75:91-102. ISENSTEIN, R.S., 1963. The life history of Cooperia onchophota (Railliet, 1898) Ransom, 1907, a nematode parasite of cattle. J. Parasitol., 49(2):235-240. KATES, K.C. & SHORB, D.A., 1943. Identification of eggs of nematodes parasitic in domestic sheep. Am. J. Vet. Res., 4:54-60. KEITH, R.K., 1953. The differentiation of the infective larvae of some common nematode parasites of cattle. Austr. J. Zool., 1/2:223-235. KHAN, Z.I: & DORSMAN, W., 1978. Trichostrongylus colubriformis: cultivation of the free-living stages.J.Parasitol., 64(6):1024-1027. KRUG, E.S. & MAYHEW, R.L., 1949. Studies on bovine gastro-intestinal parasites. XIII. Species diagnosis of nematode infections by egg characteristics. Trans. Am. Micr. Soc., 68:234-239. LEVINE, N.D.; MEHRA, K.N.; CLARK, D.T. & AVES, J., 1960. A comparison of nematode egg counting techniques for cattle and sheep feces. Am. J. ver. Res., 511-515. 47 MONNIG, H.O., 1926. The life-histories of Trichostrongylus instabilis and T. rugatus of sheep in South África.11/12 th. Rep. Vet. Res. S. Afr., 221-251. MONNIG, H.O., 1931. The specific diagnosis of nematode infestation in sheep. 17th. Rep. Ver. Res. Un. S. Afr., 221-251. MORGAN, D.O., 1930. On the differential diagnosis of larvae of some helminth parasites of sheep and goats. Jour. Helminth., 8(4) :223-228. REINECKE, R.K., 1961. III. The diagnosis of nematode parasites in ruminants for worm survey purposes. J. s. Afr. Vet. Med. Ass., 32(2:168-174. REMINGTON, R.D. & SHORK, M.A., 1970. Statistics with applications to the biological and health sciences. PrenticeHall, Inc., N.J., 418p. ROBERTS, F.H.S. & O'SULLIVAN, P.J., 1950. Methods for egg counts and larval cultures for strongyles infesting the gastro-intestinal tract of cattle. Aust. J. Agric. Res., 1(1):99-102. ROBERTS, F.H.S.; TURNER, H.N. & McKEVETT, M., 1954. On the specific distinctness of the ovine and bovine "strains" of H. contortus (Rudolphi) Cobb (Nematoda: Trichostrongylidae). Austr. J. Zool., 2:275-295. 48 SHORB, D.A., 1940. A comparative study of the eggs of various species of nematodes parasitic in domestic ruminants. J. Parasitol., 26:223-231. SILVERMAN, P.H. & CAMPBELL, J.A., 1959. Studies on parasitic worms of sheep in Scotland. I. Embrionic and larval deveIopment of Haemonchus contortus at constant conditions. Parasitology, 49(1/2):23-38. STOLL, N.R., 1923. Investigations on the control of hook-worm disease. XV. An effective method of counting hook-worm eggs in feces. Am. J. Hyg., 3:59-70. STOLL, N.R., 1930. On methods of counting nematode ova in sheep dung. Parasitology, 22:116-136. TETLEY, J.H., 1941. Haemonchus contortus eggs: comparison of those in utero with those recovered from feces, anda statistical method for identifying H. contortus in mixed infections. J. Parasitol., 27:433-463. TETLEY, J.H., 1950. The differentiation of the eggs of Haemonchus contortus and Ostertagia species of the sheep and a note on the relative generic egg-laying rates. Parasitology, 40:273-275. TURNER, J.H., 1947. Counting Nematodirus spathiger eggs in sheep dung. Proc. Helm. Soc. Wash., 18(2):132-135. 49 VEGLIA, F., 1915. The anatomy and life-history of the Haemonchus contortus (Rud.). Union So. Afr. Dept. Agr. Dir. Ver. Res. Rept., 3(4):349-500. VEGLIA, F., 1923. Preliminary notes on the life-history of Oesophagostomum columbianum. Union So. Afr. Dept. Agr. Vet. Res. Rept., 9(10):811-823. VEGLIA, F., 1928. Oesophagostomiasis in sheep (preliminary no te). 13/14th. Rep. Vet. Res. S. Afr., 755-797. WHITLOCK, H.V., 1948. Some modifications of the McMaster helminth egg-counting technique and apparatus. J. Counc. Sci. Ind. Res. Aust., 21:177-180. WHITLOCK, H.V., 1959. The recovery and identification of the first stage larvae of sheep nematodes. Austr. Ver. J.,55: 310-316. WHITLOCK, J.H., 1960. Diagnosis of veterinary parasitisms. Lea & Febiger, Philadelphia, U.S.A., 236p. ZAR, J.H., 1974. Biostatistical analysis. Prentice-Hall Inc., N.J., 620p.por
dc.rightsAcesso Abertopor
dc.subjectMedicina Veterináriapor
dc.subject.cnpqMedicina Veterináriapor
dc.titleModificação da técnica de Whitlock (1959), para o diagnóstico de nematóides gastrintestinais de bovinospor
dc.typeDissertaçãopor
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