Avaliação da curva de concentração plasmática e eficácia da ivermectina oral em cães contra Ctenocephalides felis felis e Rhipicephalus sanguineus

Abstract

getting closer and concern for the control of diseases increasing. This control is often performed with the use of chemicals known as insecticides. Among them, ivermectin is extremely widespread use because of its proven safety. Its use in dogs is suitable for the treatment of mite dermodicosi and heartworm prevention, but is extremely informally used for the control of fleas and ticks. Among the most common ectoparasites in dogs are the Rhipcephalus sanguineus tick and Ctenocephalides felis felis flea. In order to evaluate the efficacy of ivermectin against these parasites, along with treatment follow the profile of plasma tested formulation of ivermectin, a method was developed for quantification of ivermectin in canine plasma using liquid chromatography with fluorescence detection. This method was validated based on the recommendations of the National Agency of Sanitary Surveillance for validation of analytical methods and bioanalytical, Resolution RE n º 899/2003. The samples collected during the test effectiveness using this method were analyzed and showed good bioavailability with Cmax 350 ng.mL-1 in four (4) hour and at AUC of 8411. Although the formulation have shown good absorption efficacy values found for Rhipcephalus sanguineus and Ctenocephalides felis felis were very low showing this is not the most appropriate treatment for the control of these parasites.