Preparo de padrões de isoflavonas de Glycine max (L.) Merrill (soja) e contribuição na investigação fitoquímica de Dipteryx odorata (Aubl.) Willd (cumaru) - otimização de análise cromatográfica e caracterização estrutural de flavonoides

Abstract

The Glycine max (soybean) and Dipteryx odorata (tonka bean) species, belonging to the Leguminosaea family, were studied by optimizing and automating the isolation of flavonoids through high performance liquid chromatography, using a channel selector valve from Rheodyne® as fractions collector. The structural characterization was based on spectral data obtained by mass spectrometry (MS), ultraviolet (UV) and nuclear magnetic resonance (1H NMR, 13C, 1D and 2D). In the grains of Glycine max were found the flavonoids: daidzin, daidzein, glycitin, glycitein, genistin and genistein. The analysis of these compounds in soy and soy products through high performance liquid chromatography takes about 45 minutes, its quantification is dependent on the purchase of commercial analytical standards, which entails a high cost analysis. Therefore flavonoids strains were applied as standards and separation was optimized in chromatographic column of 2.4 μm, reducing in 77% the analysis time and 78% of the volume of solvent residue generated. The species Dipteryx odorata, commonly used as an herbal medicine, produces seeds with commercial value and is used in cosmetics and perfumes. In its endocarp the flavonoids identified were butin, isoliquiritigenin, luteolin, butein, sulfuretin and 3',4',7-trihydroxyflavone. The flavonois luteolin, butein and 3',4',7- trihydroxyflavone had not been previously identified in this species.