Influência dos transportadores de aminoácidos OsAAP1 e OsAAP18 na remobilização de nitrogênio e acúmulo de proteínas nos grãos de arroz

Abstract

The main goal of the study was to elucidate the functions of the OsAAP1 and OsAAP18 genes and check its influence on the N remobilization to grains filling and storage of proteins in rice plants. To this, were performed experiments to: (1) Phenotypic characterization of T-DNAi nsertional mutant plants of Dong Jin rice varietyand (2) check affinity of transport for amino acids, through functional complementation of yeast mutant strains 22 8AA, unable to transport efficiently L-proline, L-citrulline, L-aspartate and L.glutamate, 22􀀀6AAL, unable to transport efficiently L-lysine and JA248, unable to transport efficiently L-glutamine. The phenotypic analyze of the mutant plants was developed through a greenhouse experiment using theT-DNAs insertonal mutants lines 3A-00581, mutant for the gene LOC_Os07g04180 (OsAAP1), and 4A-00262, mutant for LOC_Os0636210 gene (OsAAP18) and wild type plants.Complementation tests with mutants yeasts were performed on nitrogen-free medium supplemented with L-proline, L-citrulline, L-aspartate, L-glutamate, L-lisyne or L-glutamine as the sole nitrogen source. Frm the results it was concluded that genes OsAAP1 e OsAAP18 encode to proteins involved in nitrogen remobilization to gains in rice plants and OsAAP18 influences rice productivity protein content in grains.