Atenuação do processo de lignificação em célulasde Eucalyptus urophylla S. T. Blake, em suspensão, por 2,4-D (ácido 2,4-diclorofenoxiacético)

Abstract

The suspension cell culture is an efficient tecnique to the study of secondary metabolics. Through the utilization of 2,4-D (2,4- dichlorophenoxyacetic acid), growth regulator with ability to restrain the phenylpropanoid metabolism, was carried a process of lignification of suspension cells of Eucalyptus urophylla in order to identify the most efficient 2,4-D concentration to attenuate the lignin production in vitro. This work was developed in the Wood Biotechnology Laboratory (IF-UFRRJ) where callus of Eucalyptus urophylla were produced and transfered to the medium supplemented liquid MS with five different treatments of 2,4-D (T1: 1 mg/L; T2: 2 mg/L; T3: 4 mg/L; T4: 5 mg/L; T5: 0 mg/L) without antioxidants agents. Infrared and ultraviolet analyses proved that, in medium liquid MS, the cells were capable to produce lignin. The treatment 2 with 2 mg/L of 2,4-D was better to produce little amounts of lignin than treatment 5 with 0 mg/L of 2,4-D, in which the absence of this growth regulator did not restrain the lignin production. This research opens new ways for the study of the Eucalyptus urophylla lignin allowing a better knowledge of the formation process of this substance, which has a great complexity and importance in the forest area.