Estudo histológico e localização imuno-histoquímica de proteínas do citoesqueleto em ovários, testículos e epidídimos de três espécies de lagartos da Família Leiosauridae (Reptilia: Squamata)

???item.export.label???

Please use this identifier to cite or link to this item: https://tede.ufrrj.br/jspui/handle/jspui/2467

Full metadata record

DC Field	Value	Language
dc.creator	Firmiano, Enely Maris da Silveira	-
dc.creator.Lattes	http://lattes.cnpq.br/5971248026324074	por
dc.contributor.advisor1	Pinheiro, Nadja Lima	-
dc.contributor.advisor1Lattes	http://lattes.cnpq.br/2704840396057478	por
dc.contributor.advisor-co1	Nascimento, Aparecida Alves do	-
dc.contributor.advisor-co1Lattes	http://lattes.cnpq.br/9303894913891155	por
dc.contributor.referee1	Santos, Marco Antônio José dos	-
dc.contributor.referee2	Mendes, Rosa Maria Marcos	-
dc.contributor.referee3	Santos, Clarice Machado dos	-
dc.contributor.referee4	Paoli, Severo de	-
dc.date.accessioned	2018-09-24T15:13:44Z	-
dc.date.issued	2017-08-29	-
dc.identifier.citation	FIRMIANO, Enely Maris da Silveira. Estudo histológico e localização imuno-histoquímica de proteínas do citoesqueleto em ovários, testículos e epidídimos de três espécies de lagartos da Família Leiosauridae (Reptilia: Squamata). 2017. 11 f. Dissertação (Mestrado em Biologia Animal) - Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2017.	por
dc.identifier.uri	https://tede.ufrrj.br/jspui/handle/jspui/2467	-
dc.description.resumo	Cap. I Este trabalho investiga, além da descrição histológica e histoquímica, a presença e distribuição imuno-histoquímica de alfa-actina de músculo liso, desmina e vimentina nos ovários, testículos e epidídimos de Enyalius bilineatus, Enyalius perditus e Urostrophus vautieri, representantes da família Leiosauridae, para ajudar a formar uma base de dados sobre a biologia reprodutiva destes lagartos que possam ser comparados com outras espécies de répteis, bem como com outros vertebrados. Os espécimes estudados encontram-se depositados na Coleção Herpetológica da UFJF. Os ovários, epidídimos e testículos dos animais foram removidos e encaminhados ao Laboratório de Histologia e Embriologia (UFRRJ), onde foram fixados, processados e então submetidos às técnicas histológicas, histoquímicas e imuno-histoquímicas. Os ovários destes leiosaurídeos são órgãos pareados, repletos de folículos ovarianos, em distintos estágios de desenvolvimento folicular. Estes são revestidos por epitélio cúbico simples, com uma camada de tecido conjuntivo subjacente, a túnica albugínea. Os testículos encontram-se envolvidos pela túnica albugínea e em seu interior contém septos que formam lóbulos, nos quais se localizam os túbulos seminíferos. Os epidídimos destes lagartos são ductos convolutos, revestidos internamente por um epitélio que varia de cilíndrico simples a cúbico simples com estereocílios. A imuno-histoquímica, nos ovários dos lagartos estudados, mostra uma reação moderada a acentuada para alfa-actina de músculo liso na camada da teca e em células endoteliais dos vasos sanguíneos, enquanto nos testículos destas espécies nota-se uma acentuada reação para esta proteína do citoesqueleto, na túnica albugínea, no tecido intersticial e nas células endoteliais dos vasos sanguíneos. Nos epidídimos observa-se imunomarcação para alfa-actina na cápsula e no tecido intersticial destes lagartos. Quanto ao anticorpo desmina, apenas em E. perditus, foi possível imunorreação na albugínea e no estroma ovariano, no entanto, nas três espécies analisadas, as células endoteliais dos vasos sanguíneos demonstraram reação positiva a este filamento intermediário. Nos testículos dos leiosaurídeos estudados, a desmina foi imunolocalizada na albugínea testicular e nas células endoteliais dos vasos sanguíneos, entretanto, apenas em E. bilineatus ocorreu imunomarcação, na região do interstício. Nos epidídimos, a desmina demonstrou imunorreação positiva apenas em E. perditus e E. bilineatus. Não houve reação imuno-histoquímica para vimentina nos ovários, testículos e epidídimos dos três lagartos analisados. Este estudo obteve resultados consistentes, fornecendo assim mais dados sobre a biologia reprodutiva das espécies aqui investigadas, que poderão ser utilizados para comparações filogenéticas e comportamentais com outras espécies da família Leiosauridae e outros répteis. Cap. II Este trabalho investiga, além da descrição histológica, a presença e distribuição imuno-histoquímica de alfa-actina de músculo liso, desmina e vimentina nos testículos e epidídimos de Enyalius bilineatus, Enyalius perditus e Urostrophus vautieri, representantes da família Leiosauridae, para ajudar a formar uma base de dados sobre a biologia reprodutiva destes lagartos que possam ser comparados com outras espécies de répteis, bem como com outros vertebrados. Os espécimes estudados encontram-se depositados na Coleção Herpetológica da Universidade Federal de Juiz de Fora. Os epidídimos e testículos dos animais foram removidos e encaminhados ao Laboratório de Histologia e Embriologia (UFRRJ), onde foram fixados, processados e então submetidos às técnicas histológicas e imuno-histoquímicas. O formato dos testículos varia razoavelmente entre os leiosaurídeos estudados. Estes órgãos encontram-se envoltos pela túnica albugínea e no seu interior contém septos que formam lóbulos incompletos, nos quais se localizam os túbulos seminíferos. Os epidídimos destes lagartos são ductos convolutos. Estes ductos são revestidos internamente por um epitélio que varia de cilíndrico simples a cúbico simples com estereocílios. A imuno-histoquímica revela uma acentuada reação positiva para alfa-actina de músculo liso nos testículos dos leiosaurídeos analisados, na túnica albugínea e no tecido intersticial, assim como, nas células endoteliais dos vasos sanguíneos. A desmina apresentou acentuada imunorreatividade na albugínea testicular em E. bilineatus e moderada em E. perditus e U. vautieri, entretanto, na região do tecido intersticial, apenas em E. bilineatus ocorreu imunomarcação. Nas três espécies analisadas, as células endoteliais dos vasos sanguíneos demostraram imunorreação positiva para desmina. Nos epidídimos observou-se reação imuno-histoquímica moderada para alfa-actina de músculo liso na cápsula e no tecido intersticial, enquanto a desmina demonstrou imunorreação positiva apenas em E. perditus e E. bilineatus. Não houve imunomarcação para vimentina nos testículos e epidídimos dos três lagartos analisados. Este estudo obteve resultados consistentes, fornecendo assim mais dados sobre a biologia reprodutiva das espécies aqui investigadas, que poderão ser utilizados para comparações filogenéticas e comportamentais com outras espécies da família Leiosauridae e outros répteis.	por
dc.description.abstract	Cap. I The aim of the current study is to investigate the histological and histochemical description, as well as the presence and immunohistochemical distribution of cytoskeleton proteins such as alpha smooth muscle actin, desmin and vimentin in the ovaries, testes and epididymis of Enyalius bilineatus, Enyalius perditus and Urostrophus vautieri. These species are representatives of family Leiosauridae, in order to help developing a database on the reproductive biology of these lizards, which could be compared to that of other reptile species and vertebrates. The herein studied specimens are deposited in the Herpetological Collection of Federal University of Juiz de Fora. The animals‟ ovaries, epididymis and testes were removed and sent to the Histology and Embryology Laboratory (UFRRJ), where they were fixed, processed and then subjected to histological, histochemical and immunohistochemical techniques. The ovaries of these leiosauridae are functionally-active paired organs filled with ovarian follicles at different follicular development stages. These organs are covered by a simple cubic epithelium, which presents an underlying connective tissue layer called tunica albuginea. The testes are covered by the tunica albuginea and hold septa forming lobes where the seminiferous tubules are located in. The epididymis of these lizards are convolute ducts internally coated by an epithelium, whose shape ranges from simple cylindrical to simple cubic with stereocilia. The immunohistochemical analysis applied to the ovaries of the herein studied lizards showed moderate to strong reaction to the alpha smooth muscle actin in the teak layer and in endothelial cells of blood vessels. With respect to the species‟ testes, this cytoskeleton protein showed strong reaction in the tunica albuginea, interstitial tissue, and in endothelial cells of blood vessels. As for the epididymis, the alpha-actin showed immunohistochemical reaction in the capsule and in the interstitial tissue of the lizards. The antibody „desmin‟ showed strong immunostaining in the albuginea and ovarian stroma of E. perditus, only; however, the endothelial cells in the blood vessels of the three herein analyzed species showed positive reaction to this intermediate filament. Desmin showed immunoreactivity in the testicular albuginea and in the endothelial cells of the blood vessels in the herein analyzed leiosauridae testes; however, only E. bilineatus showed immunostaining in the interstitial region. Desmin showed positive immunoreaction in the epididymis of E. perditus and E. bilineatus, only. Vimentin did not show immunostaining in the ovaries, testes and epididymis of the three analyzed lizards. The consistent results found in the current study provide additional data on the reproductive biology of the investigated species. These data may be used for phylogenetic and behavioral comparisons between the herein studied species and other species belonging to family Leiosauridae or other reptiles Cap. II The aim of the current study is to investigate the histological description, as well as the presence and immunohistochemical distribution of alpha smooth muscle actin, desmin and vimentin in the testes and epididymis of Enyalius bilineatus, Enyalius perditus and Urostrophus vautieri, which are representatives of family Leiosauridae, in order to help developing a database on the reproductive biology of these lizards, which could be compared to that of other reptile species and vertebrates. The herein studied specimens are deposited in the Herpetological Collection of Federal University of Juiz de Fora. The animals‟ epididymis and testes were removed and sent to the Histology and Embryology Laboratory (UFRRJ), where they were fixed, processed and then subjected to histological and immunohistochemical techniques. The shape of the testes varies reasonably among the studied leiosauridae. These organs are covered by the tunica albuginea and hold septa forming lobes, where the seminiferous tubules are located in. The epididymis of these lizards are convolute ducts internally coated by an epithelium, whose shape ranges from simple cylindrical to simple cubic with stereocilia. The immunohistochemical analysis applied to the testes of the herein studied lizards showed strong positive reaction to the alpha smooth muscle actin in the tunica albuginea, interstitial tissue, and in endothelial cells of blood vessels. Desmin showed strong immunoreactivity in the testicular albuginea of E. bilineatus and moderate immunoreactivity in E. perditus and U. vautieri; however, only E. bilineatus showed immunostaining in the interstitial tissue region. The endothelial cells of blood vessels showed positive immunoreaction to desmin in the three herein analyzed species. The epididymis showed moderate immunohistochemical reaction to alpha smooth muscle actin in the capsule and in the interstitial tissue, whereas desmin showed positive immunoreaction in the epididymis of E. perditus and E. bilineatus, only. Vimentin did not show immunostaining in the testes and epididymis of the three analyzed lizards. The consistent results found in the current study provide additional data on the reproductive biology of the investigated species. These data may be used for phylogenetic and behavioral comparisons between the herein studied species and other species belonging to family Leiosauridae or other reptiles.	eng
dc.description.provenance	Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2018-09-24T15:13:44Z No. of bitstreams: 1 2017 - Enely Maris da Silveira Firmiano.pdf: 12307016 bytes, checksum: d58bee7e5ddc0113fa072ab4e9aabc29 (MD5)	eng
dc.description.provenance	Made available in DSpace on 2018-09-24T15:13:44Z (GMT). No. of bitstreams: 1 2017 - Enely Maris da Silveira Firmiano.pdf: 12307016 bytes, checksum: d58bee7e5ddc0113fa072ab4e9aabc29 (MD5) Previous issue date: 2017-08-29	eng
dc.description.sponsorship	CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	por
dc.format	application/pdf	*
dc.thumbnail.url	https://tede.ufrrj.br/retrieve/8660/2017%20-%20Enely%20Maris%20da%20Silveira%20Firmiano.pdf.jpg	*
dc.thumbnail.url	https://tede.ufrrj.br/retrieve/15292/2017%20-%20Enely%20Maris%20da%20Silveira%20Firmiano.pdf.jpg	*
dc.thumbnail.url	https://tede.ufrrj.br/retrieve/21592/2017%20-%20Enely%20Maris%20da%20Silveira%20Firmiano.pdf.jpg	*
dc.thumbnail.url	https://tede.ufrrj.br/retrieve/27976/2017%20-%20Enely%20Maris%20da%20Silveira%20Firmiano.pdf.jpg	*
dc.thumbnail.url	https://tede.ufrrj.br/retrieve/34354/2017%20-%20Enely%20Maris%20da%20Silveira%20Firmiano.pdf.jpg	*
dc.thumbnail.url	https://tede.ufrrj.br/retrieve/40742/2017%20-%20Enely%20Maris%20da%20Silveira%20Firmiano.pdf.jpg	*
dc.thumbnail.url	https://tede.ufrrj.br/retrieve/47334/2017%20-%20Enely%20Maris%20da%20Silveira%20Firmiano.pdf.jpg	*
dc.thumbnail.url	https://tede.ufrrj.br/retrieve/53756/2017%20-%20Enely%20Maris%20da%20Silveira%20Firmiano.pdf.jpg	*
dc.language	por	por
dc.publisher	Universidade Federal Rural do Rio de Janeiro	por
dc.publisher.department	Instituto de Ciências Biológicas e da Saúde	por
dc.publisher.country	Brasil	por
dc.publisher.initials	UFRRJ	por
dc.publisher.program	Programa de Pós-Graduação em Biologia Animal	por
dc.relation.references	ABD-ELMAKSOUD, A. 2005. Morphological, glycohistochemical, immunohistochemical studies on the embryonic and adult bovine testis. Thesis, Institute of Veterinary Anatomy II, Faculty of Veterinary Medicine, LMU, Munich, Germany ABD-ELMAKSOUD, A. 2009. Comparative expression of laminin and smooth muscle actin in the testis and epididymis of poultry and rabbit. J Mol Hist. 40:407-416. AIRE, T. A., OZEGBE, P. C. 2007. The testicular capsule and peritubular tissue of birds: morphometry, histology, ultrastructure and immunohistochemistry. J. Anat. 210: 731-740. AIRE, T. A., OZEGBE, P. C. 2008. Immunohistochemistry of the cytoskeleton in the excurrent ducts of the testis in birds of the Galloanserae monophyly. Cell Tissue Res. 333: 311-321. AKBARSHA, M. A., KADALMANI, B., TAMILARASAN, V. 2006. Histological variation along and ultrastructural organization of the epithelium of the ductus epididymidis of the fan-throated lizard Sitana ponticeriana Cuvier. Acta Zoologica. 87:181-196. ALKAFAFY, M. 2005. Glycohistochemical, immunohistochemical and ultrastructural studies of the bovine epididymis. Thesis, Institute of Veterinary Anatomy II, Faculty of Veterinary Medicine, LMU, Munich, Germany. ANDREUCCETTI, P. 1992. An ultrastructural study of differentiation of pyriform cells and their contribution to oocyte growth in representative Squamata. Journal of Morphology. 212:1-11. ARENAS, M. I., FRAILE, B., DE MIGEL, M., PANIAGUA, R. 1995. Intermediate filaments in the testis of the teleost mosquito fish Gambusia affinis holbrooki: a light and electron microscope immunocytochemical study and Western blotting analysis. Histochemical Journal. 27: 329-337. ARENAS, M. I., BETENCOURT, F. R., FRAILE, B., PANAIGUA, R. 1997. Immunocytochemical and quantitative study of the tunica albuginea testis in young and ageing men. Histochem Cell Biol. 107: 469-477. BAKST, M. R. 1998. Structure of the avian oviduct with emphasis on sperm storage in poultry. Journal of Experimental Zoology. 282: 618-626. BANKS, F. C. L., KNIGHT, G. E., CALVERT, R. C., TURMAINE, M., THOMPSON, C. S., MIKHAILIDIS, D. P., MORGAN, R. J., BURNSTOCK, G. 2006. Smooth muscle and 96 purinergic contraction of the human, rabbit, rat, and mouse testicular capsule. Biol. Reprod. 74: 473–480. BAROUDI, R. 1970. Elementos da zoologia. 6. ed. São Paulo: Nobel. p.125-152. BÉRNILS, R. S., GIRAUDO, A. R., CARREIRA S., CECHIN, S. Z. 2007. Répteis das porções subtropical e temperada da Região Neotropical. Ciência & Ambiente. 35: 101-136. BÉRNILS, R. S., BATISTA, M. A., Bertelli, P. W. 2001. Cobras e lagartos do Vale: levantamento das espécies de Squamata (Reptilia, Lepidosauria) da Bacia do Rio Itajai, Santa Catarina, Brasil. Revista de estudos ambientais. 3: 69-79. BÉRNILS, R. S., COSTA, H. C. (org.). 2012. Brazilian reptiles: List of species. Version 2012.1. Available at http://www.sbherpetologia.org.br/. Sociedade Brasileira de Herpetologia. BERTOLUCI, J., CANELAS, M. A. S., EISEMBERG, C. C., PALMUTI, C. F. S., MONTINGELLI, G. G. 2009. Herpetofauna of Estação Ambiental de Peti, an Atlantic Rainforest fragment of Minas Gerais State, southeastern Brazil. Biota Neotrop. 9(1). BETZ, T. W. 1963. The ovarian histology of the diamond-backed water snake. Natrix rhombifera during the reproductive cycle. Journal of Morphology. 113:245-260. BOCK, P., BREITTNECKER, G. LONGLMAYR, G. 1972. Kontraktile Fibroblasten (myofibroblasten) in der lamina propria der hodenkanalchen von Menchen. Zeitschrift fur Mikroskopisch. Anatomische Forschung. 133: 519-527. BORGES-NOJOSA, D. M., CARAMASCHI, U. 2004. Composição e análise comparativa da diversidade e afinidades biogeográficas dos lagartos e anfisbenídeos (Squamata) dos brejos nordestinos. In: Ecologia e Conservação da Caatinga. Editora Universitária da UFPE, Recife. BOTTE, V., ANGELINI, F. 1980. Endocrine control of reproduction in reptiles: the refractory period. In Delrio G, Brachet J, eds. Steroids and Their Mechanisms of Action in Nonmammalian Vertebrates. New York, Raven Press, 201-212. BOYD, M. M. M. 1940. The structure of the ovary and the formation of the corpus luteum in Hoplodactylus maculatus. Q J Microsc Sci. 82:337–376. CALLEBAUT, M., VAN NASSAUW, L. 1987. Demonstration by monoclonal antidesmin of a myoid tissue coat in the preovulatory ovarian tunica albuginea of the turtle Pseudemys scripta elegans. Med. Sci. Res. 15: 1129-1130. 97 COSTA, H. C., FERNANDES, V. D., RODRIGUES, A. C., FEIO, R. N. 2009. Lizards and Amphisbaenians, municipality of Viçosa, state of Minas Gerais, southeastern Brazil. Check List. 5:3:732-745. CHAPONNIER, C., GABBIANI, G. 2004. Pathological situations characterized by altered actin isoform expression. J. Patholoy. 204:386-395. CZERNOBILSKY, B., MOLL, R., LEVY, R., FRANKE, W. W. 1985. Co-expression of cytokeratin and vimentin filaments in mesothelial, granulosa and rete ovarii cells of the human ovary. Eur. J. Cell Biol. 37: 175–190. DAVIS, J. R., LANGFORD, G A. 1970. Pharmacological studies of the testicular capsule in relation to sperm transport. In the human testis (eds Rosenberg E, Paulsen CA), 495-514. New York: Plenum. DE PÉREZ, G., RAMÍREZ M. P., CALDERÓN M. L. 1999. Estudio preliminar de la ultraestructura de la pared del ovario y de folículos previtelogénicos y vitelogénicos tempranos de Caiman crocodilus fuscus. (Reptilia, Crocodylidae). Rev. Acad. Colomb. Cienci. 23: 453-464. DE PÉREZ, G., RAMÍREZ, M. P. 2002. El tracto reproductivo de la hembra en crocodylia: Estudio histológico y ultraestructural de Caiman crocodilus fuscus (Crocodylia: Alligatoridae) Acta Biológica Colombiana. 7:1:3-19. DESANTIS, S., LABATE, M., LABATE, G. M., CIRILLO, F. 2002 Evidence of regional differences in the lectin histochemistry along the ductus epididymis of the lizard, Podarcis sicula Raf. Histochem J. 34:123-130. DIKRANIAN, K., TROSHEVA, M., PETROV, M. 1993. Intermediate filaments and ATPase activity in the vascular wall of vertebrates. Acta Histochem. 94:13-9. DINGES, H. P., ZATLOUKAL, K., SCHMID, C., MAIR, S., WIRNSBEGER, G. 1991. Co-expression of cytokeratin and vimentin filaments in rete testis and epiddiymis. Virchows Archiv. A Path. Anat. Histopath. 418:119-127. DIOKMETZIDOU, A., TSIKITIS, M., NIKOULI, S., KLOUKINA, I., TSOUPRI, E., PAPATHANASIOU, S. 2016. Chapter Fifteen-Strategies to Study Desmin in Cardiac Muscle and Culture Systems. Methods in enzymology. 568:427-459. DYCE, K. M., SACK, W. O., WENSING, C. I. G. 1997. Tratado de anatomia veterinária. Rio de Janeiro: Guanabara Koogan p. 645-647. 98 DUFAURE, J., SAINT GIRONS, H. 1984. Histologie compare de l‟epididyme et de ses secretions chez les reptiles (lezards et serpents). Archives d'anatomie microscopique et de morphologie expérimentale.73:15–26. EGGER, G. F., WITTER, K. 2009. Peritubular contractile cells in testis and epididymis of the Canis lupus familiaris. Acta Veterinaria Brno.78:3-11. ELLENPORT, C.R. Aparelho urogenital geral. 1986. In: GETTY, R. Anatomia dos animais domésticos. 5. ed. Rio de Janeiro: Guanabara Koogan. p.1813-1828. ETHERIDGE, R., WILLIAMS, E. E. 1991. A review of the South American lizard genera Urostrophus and Anisolepis (Squamata: Iguania: Polychridae). Bulletin of the Museum of Camparative Zoology. 152:317-361. FRANÇA, L.R., SILVA, JR. V.A., CHIARINI-GARCIA, H., GARCIA S. K., DEBELJUK, L. 2000. Cell proliferation and hormonal changes during postnatal development of the testis in the pig. Biol. Reprod. 63:1629-1636. FERREIRA, A., LAURA, I. A., DOLDER, H. 2002. Reproductive cycle of male green iguanas, Iguana iguana (Reptilia: Sáuria: Iguanidae), in the Pantanal of Brasil. Brazilian Journal of Morphology Science. 19:1:23-28. FERREIRA, A., SILVA, D. N., VAN SLUYS, M., DOLDER, H. 2009. Seasonal Changes in Testicular and Epididymal Histology of the Tropical Lizard, Tropidurus itambere (Rodrigues, 1987), during its Reproductive Cycle. Brazilian Journal of Biology. 69:2:429-435. FERREIRA, A.; KIHARA, V. O.; MEHANNA, M. 2011.Reproductive strategies of brazilian lizards of the genus Tropidurus Rodrigues, 1987 (Squamata, Tropiduridae) in temporal and spatial. Int. J. Morphol. 29:4: 1414-1421. FROST, D. R., ETHERIDGE, R., JAMES, D. E., TITTUS, T. A. 2001. Total evidence, sequence alignment, evolution of Polychrotid Lizards, and a reclassification of the Iguania (Squamata: Iguania). Amer. Mus. Novitates. 3343:1-38. FOX H. 1977. The urogenital system of the reptiles. In: Gans C. (Ed.), Biology of Reptilia. Academic Press, New York, p.1-127. GALOU, M., GAO, J., HUMBERT, J., MERICSKAY, M., LI, Z., PAULIN, D., VICART, P. 1997. The importance of intermediate filaments in the adaptation of tissues to mechanical stress: evidence from gene knockout studies. Biology of Cell. 89:85-97. 99 GEISLER, N., KAUFMANN, E., WEBER, K. 1982. Proteinchemical characterization of three structurally distinct domains along the protofilament unit of desmin 10 nm filaments. Cell. 30:1: 277-286. GEOGARTOS, S. P. 1993. Dynamics of intermediate filaments: recent progress and unanswered questions. FEBS. 318:101-107. GIGON-DEPEIGES, A., DUFAURE, J. P. 1977. Secretory activity of the lizard epididymis and its control by testosterone. General and Comparative Endocrinology. 33:4:473-479. GOLDBERG, S. R. 1970. Seasonal ovarian histology of the viviparous iguanid lizard Sceloporus jarrovi. Journal of Morphology. 132:265-276. GOLDBERG, S. R. 1973. Ovarian cycle of the western fence lizard, Sceloporus occidentalis. Herpetologica. 29:284-289. GOLDMAN, R. D., KHUON, S., CHOU, Y. H., OPAL, P., STEINERT, P. M. 1996: The function of intermediate filaments in cell shape and cytoskeletal integrity. J. Cell Biol. 134: 971-983. GRIBBINS, K. M., GIST, D. H. 2003. Cytological evaluation of spermatogenesis within the germinal epithelium of the male european wall lizard, Podarcis muralis. Journal of Morphology. 258:296-306. GRIBBINS, K. M., GIST, D. H., CONGDON, J. 2003. Cytological evaluation of spermatogenesis in the red-eared slider. Trachemys scripta. Journal of Morphology. 255:337-346. GRIBBINS, K. M., ELSEY, R. M., GIST, D. H. 2006. Cytological evaluation of the germ cell development strategy within the testes of the American alligator, Alligator mississippiensis, Acta Zoology. 87:59-69. GRIBBINS, K. M., MILLS, E. M., SEVER, D. 2007. Ultrastructural examination of spermiogenesis within the testis of the ground skink, Scincella laterale (Squamata, Sauria, Scincidae), Journal of Morphology. 268:181-192. GRIBBINS, K., RHEUBERT, J., COLLIER, M., SIEGEL, D., SEVER, D. 2008. Histological analysis of spermatogenesis and the germ cell development strategy within the testis of the male western Cottonmouth Snake, Agkistrodon piscivorus. Annals of Anatomy. 190:461-476. GRIBBINS, K. M. 2011. Reptilian spermatogenesis: a histological and ultrastructural perspective. Spermatogenesis. 3:250–269. 100 GRIBBINS, K. M., MATCHETT, C. L., DELBELLO, K. A., RHEUBERT, J., VILLAGRÁN-SANTACRUZ, M., GRANADOS-GONZÁLEZ, G., HERNÁNDEZ-GALLEGOS, O. 2014. The ultrastructure of spermatid development during spermiogenesis within the rosebelly lizard, Sceloporus variabilis (Reptilia, Squamata, Phrynosomatidae). Journal of Morphology. 275:258–268. GRIER, H. J., URIBE, M. C, LO NOSTRO, F. L., MIMS, S. D., PARENTI, L. R. 2016. Conserved form and function of the germinal epithelium through 500 million years of vertebrate evolution. Journal of Morphology. 277:8:1014-44. GÓMEZ, D., RAMÍREZ-PINILLA, M. P. 2004. Ovarian Histology of the Placentotrophic Mabuya mabouya (Squamata, Scincidae). Journal of Morphology 259:90-105. GURAYA, S. S. 1989. Ovarian follicles in reptiles and birds. Springer-Verlag, Berlin: 287 p. HAHN, W. E. 1964. Seasonal changes in testicular and epididymal histology and spermatogenic rate in the lizard Uta stansburiana stejnegeri. Journal of Morphology. 115:3:447-459. HAMMOUCHE, S., GERNIGON, T., EXBRAYAT, J. M. 2009. Correlation between ovarian steroidogenesis and beta-endorphin in the Lizard Uromastyx acanthinura: Immunohistochemical approach. Folia Histochem Cytobiol. 47:5:S95-S100. HARGROVE, J. L., MACINDOE, J. H., ELLIS, L.C. 1977. Testicular contractile cells and sperm transport. Fertil Steril. 28:146-1157. HECKMANN, M., BAUER, R., JUNGMANN, A., WINTER, L., RAPTI, K., STRUCKSBERG, K. 2016. AAV9-mediated gene transfer of desmin ameliorates cardiomyopathy in desmin-deficient mice. Gene therapy. 23:8-9:673-9. HELFAND, B. T., CHANG, L., GOLDMAN, R. D. 2003. The dynamic and motile properties of intermediate filaments. Annual Review of Cell Developmental Biology. 19:445-467. HELFAND, B., CHANG, L., GOLDMAN, R. 2004. Intermediate filaments are dynamic and motile elements of cellular architecture. Cell science. 117:2:133-141. HENDERSON, D., WEBER, I. C. 1980. Immunoelectron microscopic studies of intermediate filaments in culture cells. Expl Cell Res. 129:441-53. HESS, R. A., THURSTON, R. J., BIELLER, H. V. 1976. Ultrastructure of the epididymal region and ductus deferens of the turkey (Meleagris gallopavo). J. Anat. 122:241-252. 101 HERRMANN, H., HESSE, M., REICHENZELLER, M., AEBI, U. 2003. Functional complexity of intermediate filament cytoskeletons: from structure to assembly to gene ablation. Int Rev Cytol. 223:83-175. HERRMANN, H., AEBI, U. 2004. Intermediate filaments: molecular structure, assembly mechanism, and integration into functionally distinct intracellular scaffolds, Annu Rev Biochem. 73:749-89. HERNÁNDEZ-GALLEGOS O., MÉNDEZ-DE LA CRUZ, F.M. R., VILLAGRÁN-SANTACRUZ, M., RHEUBERT, J. L., GRANADOS-GONZÁLEZ, G., GRIBBINS, K. M. 2014. Seasonal spermatogenesis in the Mexican endemic oviparous lizard, Sceloporus aeneus (Squamata: Phrynosomatidae). Spermatogenesis. 4:3: 988585. HERNÁNDEZ-FRANYUTTI A., URIBE ARANZÁBAL M. C., GUILLETTE L. J. JR. 2005. Oogenesis in the viviparous matrotrophic lizard Mabuya brachypoda. Journal of Morphology 265:152-164. HILDEBRAND, M. Análise da estrutura dos vertebrados. São Paulo: Atheneu, 1995. p. 700. HINZ, B. 2007. Formation and function of the myofibroblast during tissue repair. J Invest Dermatol. 127:526-537. HOLSTEIN, A. F., MAEKAWA, M., NAGANO, T. 1996. Myofibroblasts in the lamina propria of human seminiferous tubules are dynamic structures of heterogeneous phenotype. Arch Histol Cytol. 59:109-125 HONDO, E., KITAMURA, N., TORIBA, M., KUROHMARU, M., HAYASHI, Y., YAMADA, J. 1997. Histological study of the seminiferous epithelium in the Japanese rat snake, Elaphe climacophora: identifation of spermatogonium. Journal of Veterinary Medical Science 59:23-29. HUBERT, J. 1985. Origin and development of oocytes. In: Gans C, editor. Biology of the Reptilia. New York: John Wiley & Sons. 14:41-74. IVASKA, A. J., PALLARIB, H-M., NEVOA, J., ERIKSSON, J. E. 2007. Novel functions of vimentin in cell adhesion, migration, and signaling. Exp Cell Res. 313:10:2050-62. JACKSON, J. F. 1978. Differentiation in the genera Enyalius and Strobilurus (Iguanidae): implications for Pleistocene climatic changes in eastern Brazil. Arquivos de Zoologia. 30: 1-40. 102 JEZEK, D., HITTMAIR, A., ROGATSCH, H. 1996. Lamina propria of sex cords in human fetal testis: an immunohistologial and stereological study. Anatomy and Embryology. 193:181-190. JONES, R. E. 1987. Ovulation: insights about the mechanisms based on a comparative approach. En D. O. Norris, R. E. Jones (eds.). Hormones and reproduction in fishes, amphibians and reptiles, 203-240. New York: Plenum Press. JONES, R. E., SWAIN, T., GUILLETTE, L. J., FITZGERALD, K. T. 1982. The comparative anatomy of lizard ovaries, with emphasis on the number of germinal beds. J Herpetol 16:240-252. JUNQUEIRA, L. C., CARNEIRO, J. 2013. Histologia Básica. 12ªed. Rio de Janeiro: Guanabara Koogan. 538p. KABSCH, W., VANDEKERCKHOVE, J. 1992. Structure and function of actin. Annu Rev Biophys Biomol Struct. 21:49-76. KASPER, M., P. STOSIEK. 1989. Immunohistochemical investigation of different cytokeratins and vimentin in the human epididymis from the fetal period up to adulthood. Cell Tissue Res. 257:661-664. KHAN-DAWOOD, F. S., DAWOOD, M. Y., TABIBZADEH, S. 1996. Immunohistochemical analysis of the microanatomy of primate ovary. Biol. Reprod. 54:734-742. KIERSZENBAUM, A. L. Histologia e Biologia Celular - uma introdução à patologia. 3ª Edição. Rio de Janeiro: Elsevier, 2012. 704p. KINNER, B., ZALESKAS, J. M., SPECTOR, M. 2002. Regulation of smooth muscle actin expression and contraction in adult human mesenchymal stem cells. Exp Cell Res. 278:72-83. KLOSTERMAN, L. L. 1983. The ultrastructure of germinal beds in the ovary of Gerrhonotus coeruleus (Reptilia: Anguidae). Journal of Morphology. 178:247-266. LAZARIDES, E. 1980. Intermediate filaments as mechanical integrators of cellular space. Nature 283:249-256. LAZARIDES, E. 1982. Biochemical and immunocytological characterization of intermediate filaments in muscle cells. Methods Cell Biol. 25:333-57. LEESON, C. R., FORMAN, D. E. 1981. Postnatal development and differentiation of contractile cells within the rabbit testis. J Anat. 132: 491-511. 103 LYALL, F., ROBSON, S. C., BULMER, J. 2013. Spiral artery remodeling and trophoblast invasion in preeclampsia and fetal growth restriction relationship to clinical outcome. Hypertension. 2:6:1046-1054. LIANG, G., LIU, Q., YU, H., WANG, Q. 2011. Histological and immunocytochemical study of deferens ducts in the Chinese rat snake (Zaocys dhumnades). Zoological Research. 329:6:663-669. LIMA, A. F. B., SOUSA, B. M. 2006. Court and copulation behaviors of Enyalius perditus Jackson, 1978 (Squamata, Leiosauridae) in captivity conditions. Revista Brasileira De Zoociências. 8:2:193-197. LOREDANA, R., VACCARO, M. C., PRISCO, M., CAROTENUTO, R., LIGUORO, A., ANDREUCCETTI, P. 2004. a and b Spectrin distribution during the differentiation of pyriform cells in follicles of lizard Podarcis sicula. Molecular Reproduction and Development. 67:101-107. LOURENÇO, L. 2008. Protocolos de rotina do laboratório de histologia e anatomia patológica. UTAD. Ed, Universidade de Trás-os-montes e Alto Douro. Vila Real. LUNA, E. J., HITT, A. L. 1992. Cytoskeleton-plasma membrane interactions. Science. 258: 955-963. MACHADO-SANTOS, C., SANTANA, L. N. S., VARGAS, R. F. G., ABIDU-FIGUEIREDO, M., BRITO-GITIRANA, L.,. CHAGAS, M. A. 2015. Histological and immunohistochemical study of the ovaries and oviducts of the juvenile female of Caiman latirostris (Crocodilia: Alligatoridae). Zoologia (Curitiba) [online]. 32:5: 395-402. MADEKUROZWA, M. C., KIMARO, W. H. 2006. A morphological and immunohistochemical study of healthy and atretic follicles in the ovary of the sexually immature ostrich (Struthio camelus). Anat. Histol. Embryol. 35: 253-258. MADEKUROZWA, M. C. 2007. An immunohistochemical study of the distribution of intermediate filaments in the ovary of the emu (Dromaius novaehollandiae). Anat. Histol. Embryol. 36: 336-342. MAEKAWA, M., NAGANO, T., KAMIMURA, T., ISHIKAWA, H., DEZAWA, M. 1991. Distribution of actin-filament bundles in myoid cells, Sertoli cells, and tunica albuginea of rat and mouse testes. Cell Tissue Res 266: 295–300. MAHMOUD, I. Y., CYRUS, R. V., BENNETT, T. M., WOLLER, M. J., MONTAG, D. M. 1985. Ultrastructural changes in testes of the snapping turtle, Chelydra serpentina in 104 relation to plasma testosterone, Δ 5-3β-hydroxysteroid dehydrogenase, and cholesterol. General and comparative endocrinology, 57:3:454-464. MANES, M. E., NORIEGA, T., CASAL, F. C., APICHELA, S. 2007. Ovarian changes during the reproductive cycle of the Tupinambis merianae lizard raised in a temperate environment. Cuadernos de Herpetologia 21: 21-29. MARETTA, M., MARETTOVA, E. 2004. Immunohistochemical demonstration of myoid cells in the testis and its excurrent ducts in the domestic fowl. Br Poultry Sci 45: 585–589. MARETTOVA, E., MARETTA, M. 2002. Demonstration of intermediate filaments in sheep ovary. Acta Histochemica, 104:431-434. M MAURIZII, M. G., TADDEI, C. 1996. Immunolocalization of cytoskeletal proteins in the previtellogenic ovarian follicle of the lizard Podarcis sicula. Cell Tissue. 284:489-493. MENDES, R. M. M., PINHEIRO, N. L., NASCIMENTO, A. A., SANTOS, C. M., RIBEIRO, T. P., SANTOS, M. A. J., SALES, A. 2009. Histologia comparada de testículo e do segmento sexual do rim de lagarto tropical Tropidurus torquatus Wied, 1820 (Squamata: Tropiduridae) adultos e imaturos. Revista Universidade Rural. Série Ciências da Vida. 29:49-54. MESURE, M., CHEVALIER, M., DEPEIGES, A., FAURE, J., DUFAURE, J. P. 1991. Structure and ultrastructure of the epididymis of the viviparous lizard during the annual hormonal cycle: Changes of the epithelium related to secretory activity. J Morphol 210:133–145. MIETTINEN, M., VIRTANEN, I., TALERMAN, A. 1985. Intermediate filament proteins in human testis and testicular germ cell tumours. Am. I. Pathol. 120: 402-10. MOODLEY, G. K., VAN WYK, J. H. 2007. Folliculogenesis and ovarian histology of the oviparous gecko, Hemidactylus mabouia (Sauria: Gekkonidae). African Journal of Herpetology 56: 115-135. MOORE, R., CLARK, D., VODOPICH, D. S. 1998 - Botany - McGraw-Hill Education, 832 p. MOTTA, C. M., CASTRIOTA-SCANDERBEG, M., FILOSA, S., ANDREUCCETTI, P. 1995. Role of pyriform cells during the growth of oocytes in the lizard Podarcis sicula. J Exp Zool 273:247–256. 105 MOTTA, C. M., FILOSA, S., ANDREUCCETTI, P. 1996. Regression of the epithelium in the late previtellogenic follicles of Podarcis sicula: a case of apoptosis. J Exp Zool 276:233–241. MCHUGH, K. M., CRAWFORD, K., LESSARD, J. L. 1991. A Comprehensive Analysis of the Developmental and Tissue-Specific Expression of the Isoactin Multigene Family in the Rat. Developmental Biology 148:442-458. NEAVES, W. B. 1971. Intercellular bridges between follicle cells and oocyte in the lizard Anolis carolinensis. Anat Rec 170: 285–302. NOBLE, G. A., NOBLE, E. R. 1940. A brief anatomy of turtle. Stanford University Press, Stanford University California. London: Hamphrey Oxford University Press, 49p. NORRIS, D. O., JONES, R. E. 2012. Hormones and reproduction in fishes, amphibians, and reptiles. Springer Science & Business Media. 613 p. ORR, R. T. Biologia dos vertebrados. 5. ed. São Paulo: Livraria Rocca Ltda., 1986. p. 508. ORTEGA, H. H., SALVETTI, N. R., MÜLLER, L. A., AMABLE, P., LORENTE, J. A., BARBEITO, C. G., GIMENO, E. J. 2007. Characterization of cytoskeletal proteins in follicular structures of cows with cystic ovarian disease. Journal of comparative pathology, 136:4: 222-230. O'SHEA, J., ROBSON, R., HARTZER, M., HUIATT, T., RATHBUN, W., STROMER, M. 1981. Purification of desmin from adult mammalian skeletal muscle. Biochemical Journal, 195:2: 345-356. OZEGBE, P. C., KIMARO, W., MADEKUROZWA, M. C., SOLEY, J. T., AIRE, T. A. 2010. The Excurrent Ducts of the Testis of the Emu (Dromaius novaehollandiae) and Ostrich (Struthio camelus): Microstereology of the Epididymis and Immunohistochemistry of its Cytoskeletal Systems. Anat. Histol. Embryol. 39:7–16. PALOMBI, F., FARINI, D., SALANOVA, M. DE GROSII, S., STEFANIN, M. 1992. Development and cytodifferentiation of peritubular myoid cells in the rat testis. Anatomical Record, 233: 32-41. PARANKO, J., PELLINIEMI, L. J. 1992. Differentiation of smooth muscle cells in the fetal rat testis and ovary: localization of alkaline phosphatase, smooth muscle myosin, F-actin, desmin. Cell Tissue Res 268: 521–530 PAULIN, D., LI, Z. 2004. Desmin: a major intermediate filamento protein essential for the structural integrity and function of muscle. Exp. Cell Res. 301: 1–7. 106 PFEIFFER, D. C., VOGL, A.W. 1992. Actin filaments associated with the basal Sertoli cell surface in the alligator and turtle. Tissue Cell. 24:5:643-54. PELLINIEMI, L. J., KUOPIO, T., FROJDMAN, K. 1996. The cell biology and function of the fetal Leydig cell. The Leydig cell. Cache River Press, Vienna, IL, 143-158. PERRY, M. M., GILBERT, A. B., EVANS, A. J. 1978. Electron microscope observations on the ovarian follicle of the domestic fowl during the rapid growth phase. Journal of Anatomy, 125:481-497. PIRES, M., COELHO, A. C., PALMEIRA, C., CARDOSO, M. N., SEIXAS, F., RODRIGUES, J. 2010. Protocolos de Imunologia. 49-55. POUGH, F. H.; JANIS, M. C.; HEISER, B. J. 2003. A vida dos vertebrados. 3. ed. São Paulo: Atheneu. p. 270-290. RAMAEKERS, F. C. S., FEITZ, W., MOESKE, O., SCHAART, G., HERMAN, C., DEBRUYNE, F., VOOIJES, G. P. 1985. Antibodies to cytokeratin and vimentin in testicular tumour diagnosis. Virchows Arch. A Pathol. Anat. 408: 127-142. RAMÍREZ-PINILLA, M. P., DE PÉREZ, G., RAMÍREZ-PERILLA, J. 1989. Histologıa del tracto reproductivo de la hembra del lagarto Phenacosaurus heterodermus (Reptilia: Sauria: Iguanidae). Trianea 3: 93–103. RAHIL, K. S. E NARBAITZ, R. 1973. Ultrastructural studies in the relationship between follicular cells and growing oocytes in the turtle Pseudemys scripta. Journal of Anatomy 115:175-186. RAUCCI, F., DI FIORE, M. M. 2010. The maturation of oocyte follicular epithelium of Podarcis s. sicula is promoted by d-aspartic acid. Journal of Histochemistry and Cytochemistry. 58:2:157-171. REDMER, D. A., V. DORAISWAMY, B. J. BORTNEM, K. FISHER, A. JABLONKA-SHARIFF, A. T. GRAZUL-BILSKA, L. P. REYNOLDS. 2001. Evidence for a role of capillary pericytes in vascular growth of the developing ovine corpus luteum. Biol. Reprod. 65: 879–889. RIBEIRO, L. B., SOUSA, B. M. 2006. Urostrophus vautieri (NCN). Skin shedding. Herpetological Review 37:3: 348. RODRIGUES, M. T., FREITAS, M. A., SILVA, T. F. S., BERTOLOTTO, C. E. V. 2006. A new species of lizard genus Enyalius (Squamata: Leiosauridae) from the highlands of Chapada Diamantina, state of Bahia, Brazil, with a key to species. Phyllomedusa, 5: 11-24. 107 ROGATSCH, H., JEZEK, D., HITTMAIR, A., MICUZ, G., FEICHTINGER, H. 1996. Expression of vimentin, cytokeratin, and desmin in Sertoli cells of human fetal, cryptorchid, and tumour-adjacent testicular tissue. Virchows Arch A Pathol Anat 427:497-502. ROSSI, F., FERRARESI, A., ROMAGNI, P., SILVESTRONI, L., SANTIEMMA, V. 2002. Angiotensin II stimulates contraction and growth of testicular peritubular myoid cells in vitro. Endocrinology. 143:8: 3096-3104. ROTHWELL, B., SOLOMON, S. E. 1977. The ultrastructure of the follicle wall of the domestic fowl during the phase of rapid growth. British Poultry Science, 18: 605-610. ROTHWELL, B., TINGARI, M. D. 1973. The ultrastructure of the boundary tissue of the seminiferous tubule in the testis of the domestic fowl (Gallus domesticus). J Anat 114: 321–328. RHEUBERT, J. L., TOUZINSKY, K., SEVER, D. M., ALDRIDGE, R. D., WILMES, A. J., SIEGEL, D. S., GRIBBINS, K. M. 2014. Reproductive Biology of Sceloporusconsobrinus (Phrynosomatidae): Male Germ Cell Development and Reproductive Cycle Comparisons within Spiny Lizards. Journal of Herpetology. 48:2:162-17. RUSSEL, L. D., SINHA-HIKIM, A. P., GHOSH, S., BARTKE, A. 1994. Structure-function relationships in somatic cells of the testis and accessory reproductive glands. In: BARTKE A. (eds). Function of somatic cells in the testis. Norwell: Spring-Verlag. p55-83. SADJIA, H., GERNIGON SPYCHALOWICZ, T., EXBRAYAT, J. M. 2007. Immunolocalization of estrogens and progesterone receptors within the ovary of the lizard Uromastyx acanthinura from vitellogenesis to rest season. Folia Histochemica et Cytobiologica. 45: 23-27. SANDLEIR, R. M. F. S. 1973. The reproduction of vertebrates. New York: Academic press. p. 56-59. SANTOS, R. C., LUCAS, P. S., SOUSA, B. M., NOVELLI, I. A. 2009. Reptilia, Squamata, Leiosauridae, Urostrophus vautieri: Distribution extension and geographic distribution map. Check List. 5:3: 533–536. SANTOS, H. S. et al. 2015. Ovarian follicular cycle of Tropidurus hispidus and Tropidurus semitaeniatus (Squamata: Tropiduridae) in a semiarid region of Brazil. Zoologia. 32:1: 86-92. 108 SAZIMA, I., HADDAD, C. F. B. 1992. Répteis da Serra do Japi: notas sobre história natural. In História natural da Serra do Japi: ecologia e preservação de uma área florestal no sudeste do Brasil. (P. Morellato, org.). Editora da UNICAMP, Campinas, 212-235. SCHAFFELD, M., HERRMANN, H., SCHULTESS, J., MARKL, J. 2001. Vimentin and desmin of a cartilaginous fish, the shark Scyliorhinus stellaris: sequence, expression patterns and in vitro assembly. Eur J Cell Biol. 80: 692-702. SCHLATT, S., WEINBAUER, G. F., ARSLAN, M., NIESCHLAG, E. 1993. Appearance of α-smooth muscle actin in peritubular cells of monkey testes is induced by androgens, modulated by follicle-stimulating hormone, and maintained after hormonal withdrawal. J Androl. 14: 340-350 SELSTAM, G., I. NILSSON, AND M. O. MATTSSON. 1993. Changes in the ovarian intermediate filament desmin during the luteal phase of the adult pseudopregnant rat. Acta Physiol. Scand. 147: 123-129. SEVER D. M. 2010. Ultrastructure of the reproductive system of the black swamp snake (Seminatrix pygaea). VI. Anterior testicular ducts and their nomenclature. Journal of Morphology. 271:104-115. SEVER, D. M., FREEBORN, L. R. 2012. Observations on the anterior testicular ducts in snakes with emphasis on sea snakes and ultrastructure in the yellow-bellied sea snake, Pelamis platurus. Journal of Morphology. 273: 324-336. SIEGEL, D. S., SEVER, D. M., RHEUBERT, J. L., GRIBBINS, K. M. 2009. Reproductive biology of Agkistrodon piscivorus Lacépède (Squamata, Serpentes, Viperidae, Crotalinae) Herpetol Monographs. 23:74–107. SINGH, D. K., MOHANTY, N., JENA, N. 2016. Reproductive cycle of male common Indian rock lizard, Psammophilus blanfordanus: Seasonal testis, epididymis and renal sex segment histology. The Journal of Zoology Studies, 3:6: 45-55. SILVA, D., RODRIGUES, M., MEHANNA, M., FERREIRA, A., DOLDER, H. 2016. Células germinativas e espermatogênese do lagarto Tropidurus torquatus (Tropiduridae) de uma área urbana no bioma cerrado do centro-oeste brasileiro. Bioscience Journal. 32:6:1595-1603. SILVEIRA, J. A. 2009. Histologia testicular e caracterização dos estádios do ciclo do epitélio seminífero de Hemidactylus mabouia, (Moreau De Jonnès, 1818) (Reptilia, Squamata, 109 Sauria, Gekkonidae). Dissertação (mestrado em biologia celular e estrutural). Universidade Federal De Viçosa, Minas Gerais, Brasil. 60p. SKALLI, O., ROPRAZ, P., TRZECIAK, A., BENZONANA, G., GILLESSEN, D., GABBIANI, G. 1986. A monoclonal antibody against -smooth muscle actin: A new probe for smooth muscle differentiation. J Cell Biol. 103:2787-2796. SOUSA, B. M. 2000. Aspectos ecológicos, comportamentais e morfológicos associados à alimentação de Enyalius perditus Jackson, 1978 (Sauria: Polychrotidae). Tese de doutorado, UFRRJ, 168p. STEGER, K., WROBEL, K. H. 1994. Immunohistochemical demonstration of cytoskeletal proteins in the ovine testis during postnatal development. Anatomy and Embryology. 189: 521-530. STORER, T. I., USINGER, R. L., STEBBINS, R. C., NYBAKKEN, J. W. 2000. Zoologia geral. 6 ed. v. 8. São Paulo: Companhia Editora Nacional, p. 642-654. TAYLOR, C. 2014. Predictive Biomarkers and Companion Diagnostics. The Future of Immunohistochemistry - „in situ proteomics‟, or just a „stain‟? Appl Immunohistochem Mol Morphol. 22:8: 555-561. TEIXEIRA, R. L., ROLDI, K., VRCIBRADIC, D. 2005. Ecological Comparisons between the Sympatric Lizards Enyalius bilineatus and Enyalius brasiliensis (Iguanidae, Leiosaurinae) from an Atlantic Rain-Forest Area in Southeastern Brazil. Journal of Herpetology. 39:3: 504-509. TUMKIRATIWONG, P., MEESUK, W., CHANHOME, L., AOWPHOL, A. 2012. Reproductive patterns of captive male and female monocle cobra, Naja kaouthia (Lesson, 1831). Zoological Studies. 51:5: 692-700. UETZ, P., JIRÍ HOŠEK (eds.), The Reptile Database, http://www.reptile-database.org, acessado em abril, 2017. URIBE, M. C., OMANA, M. E. M., QUINTERO, J. E. G., GUILLETTE, L. J. JR. 1995. Seasonal variation in ovarian histology of the viviparous lizard Sceloporus torquatus torquatus. Journal of Morphology 226:103-119. URIBE, M. C., PORTALES, G., GUILLETTE, L. J. JR. 1996. Ovarian folliculogenesis in the oviparous Mexican lizard Ctenosaura pectinata. Journal of Morphology. 230: 99-112. URIBE, M. C. A., GUILLETTE, L. J. 2000. Oogenesis and ovarian histology of the American alligator Alligator mississippiensis. Journal of Morphology. 245:3:225-240. 110 VAN DEN HURK, R., G. DIJKSTRA, F. N. VAN MIL, S. C. J. HULSHOF, AND S. G. A. M. 1995. Distribution of the intermediate filament proteins vimentin, keratin and desmin in the bovine ovary. Mol. Reprod. Dev. 41: 459-467. VAN NASSAUW, L., CALLEBAUT, M., HARRISSON, F., DANEELS, G., MOEREMANS, M. 1989. Immunohistochemical localization of desmin in the quail ovary, demonstration of a suspensory apparatus. Histochemistry. 90: 371-377. VAN NASSAUW, L., CALLEBAUT, M. 1991. Structural and immunohistochemical aspects of the postovulatory follicle in Japanese quail. Anatomical Records. 229: 27-30. VAN NASSAUW, L., F. HARRISSON, CALLEBAUT, M. 1991: Localization of smooth-muscle markers in the ovaries of some ectothermic vertebrates. Anatomical Records. 229: 439-446. VAN NASSAUW, L., HARRISSON, F., CALLEBAUT, M. 1992. Immunolocalization of smooth muscle-like cells in the quail ovary. European Journal of Morphology. 30: 275-288. VAN NASSAUW, L., CALLEBAUT, M., HARRISSON, F., SCHEUERMANN, D. W. 1992. Smooth muscle cells in the walls of ovarian follicles in the Japanese quail. Cell Tissue Res. 269: 49-56. VAN NASSAUW, L., HARRISON, F., CALLEBAUT, M. 1993. Smooth muscle cells in the peritubular tissue of the quail testis. European Journal of Morphology. 31: 60-64. VAN VORSTENBOSCH, C. J., SPEK, E., COLENBRANDER, B., WENSING, C. J. 1984. Sertoli cell development of pig testis in the fetal and neonatal period. Biol. Reprod. 31: 565-77. VAN WYK, J. H. 1984. Ovarian morphological changes during the annual breeding cycle of the rock lizard Agama atra (Sauria: Agamidae). Navorsinge van die Nasionale Museum Bloemfontein, Bloemfontein. 4: 237-275. VAN WYK, J. H. 1995. The male reproductive cycle of the lizard Cordylus giganteus (Sauria: Cordylidae). J. Herpetol. 29: 522-535. VIEIRA, G.H.C., WIEDERHECKER, H.C., COLLI, G.R., BAO, S.N. 2001. Spermiogenesis and testicular cycle of the lizard Tropidurus torquatus (Squamata, Tropiduridae) in the Cerrado of central Brazil. Amphibia-Reptilia. 22: 217- 223. 111 VIEIRA, S., ROMERO DE PEREZ, G., RAMÝREZ-PINILLA, M. P. 2010. Ultrastructure of the ovarian follicles in the placentotrophic Andean Lizard of the genus Mabuya (Squamata: Scincidae). Journal of Morphology. 271:6:738-749. VIRTANEN, I., KALLAJOKI, M., NARVANEN, O., PARENKO, J., THORNELL, L.E., MIETTINEN, M., LEHTO, V.P. 1986. Peritubular myoid cells of human and rat testis are smooth muscle cells that contain desmin-type intermediate filaments. Anatomical Record. 215:10-20. VITT, L. J., ÁVILA-PIRES, T. C. S., ZANI, P. 1996. Observations on the ecology of the rare Amazonian lizard Enyalius leechii (Polychrotidae). Herpetological Natural History. 4:77-82. WAKUI, S., M. FURUSATO, S. USHIGOME, Y. KANO, 1994. Co-expression of different cytokeratins, vimentin and desmin in the rete testis and epididymis in the dog. Journal of Anatomy. 184:147-151. WROBEL, K-H., DOSTAL, S., SCHIMMER, M. 1988. Postnatal development of the tubular lamina propria and the intertubular tissue in the bovine testis. Cell Tissue Res. 252: 639-653. YOKOI, Y., NAMIHISA, T., KURODA, H., KOMATSU, I., MIYAZAKI, A., WATANABE, S., USUI, K. 1984. Immunocytochemical detection of desmin in fat-storing cells (Ito cells). Hepatology. 4: 709-14. YOSHIMURA, Y., TANAKA, K., KOGA, O. 1983. Studies on the contractility of follicular wall with special reference to the mechanism of ovulation in hens. British Poultry Science. 24: 213-218. ZAMPROGNO, C., ZAMPROGNO, M. G. F., TEIXEIRA, R. L. 2001. Evidence of terrestrial feeding in the arboreal lizard Enyalius bilineatus (Sauria, Polychrotidae) of South-Eastern Brazil. Rev. Bras. Biol. 61: 91-94. ZATZ, M. G. 2002. O polimorfismo cromático e sua manutenção em Enyalius sp (Squamata: Leiosauridae) no Cerrado do Brasil Central. Dissertação de Mestrado, Universidade de Brasília, Brasília, DF. ZHU, L. J., S. D. ZONG, D. M. PHILLIPS, A. J. MOO-YOUNG, C. W. BARDIN, 1997. Changes in the distribution of intermediate filaments in the rat Sertoli cells during the seminiferous epithelium cycle and postnatal development. Anat. Rec. 248: 391–405. ZUG, G. R., VITT, L. J., CALDWELL, J. P. 2001. Herpetology: An introductory biology of amphibians and reptiles. Academic Press: New York. 3rd ed. 697 p.	por
dc.rights	Acesso Aberto	por
dc.subject	Imunorreação	por
dc.subject	morfologia	por
dc.subject	Squamatas	por
dc.subject	sistema reprodutor	por
dc.subject	Imunorreação	por
dc.subject	répteis	por
dc.subject	microfilamentos	por
dc.subject	filamentos intermediários	por
dc.subject	Immunoreactivity	eng
dc.subject	morphology	eng
dc.subject	Squamates	eng
dc.subject	reproductive system	eng
dc.subject	Immunoraction	eng
dc.subject	reptiles	eng
dc.subject	microfilaments	eng
dc.subject	intermediate filaments	eng
dc.subject.cnpq	Zoologia	por
dc.title	Estudo histológico e localização imuno-histoquímica de proteínas do citoesqueleto em ovários, testículos e epidídimos de três espécies de lagartos da Família Leiosauridae (Reptilia: Squamata)	por
dc.title.alternative	Histological study and localization immunohistochemical of cytoskeleton proteins in testes and epididymis of three lizard species belonging to family Leiosauridae (Reptilia: Squamata)	eng
dc.type	Tese	por
Appears in Collections:	Doutorado em Biologia Animal

Files in This Item:

File	Description	Size	Format
2017 - Enely Maris da Silveira Firmiano.pdf	Documento principal	12.02 MB	Adobe PDF	Download/Open Preview ×

Show simple item record Recommend this item

Universidade Federal Rural do Rio de Janeiro

???jsp.home.dspace.heading.type???