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dc.creatorVasconcelos Filho, Wilson Franklim de-
dc.creator.Latteshttp://lattes.cnpq.br/9810757771601517por
dc.contributor.advisor1Mello, Marco Roberto Bourg de-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/3560332978218414por
dc.contributor.referee1Dias, Angelo José Burla-
dc.contributor.referee2Jesus, Vera Lúcia Teixeira de-
dc.date.accessioned2017-07-24T12:38:24Z-
dc.date.issued2010-03-05-
dc.identifier.citationVASCONCELOS FILHO, Wilson Franklim de. Eficiência da congelação automatizada na viabilidade de sêmen bovino. 2010. 65 f. Dissertação (Programa de Pós-Graduação em Zootecnia) - Universidade Federal Rural do Rio de Janeiro, Seropédica.por
dc.identifier.urihttps://tede.ufrrj.br/jspui/handle/jspui/1903-
dc.description.resumoMesmo com os avanços tecnológicos no campo da inseminação artificial, ainda é grande o número de pessoas que realizam a congelação de sêmen de maneira manual. Esta técnica tem se mostrado viável, porém, é difícil padronizar as curvas de resfriamento e de congelação, uma vez que dependem da qualidade do material utilizado (vedação da geladeira, tipo de caixa de isopor, quantidade de gelo, nível de nitrogênio líquido e outros). Portanto, o objetivo deste estudo foi avaliar o efeito da criopreservação de sêmen bovino utilizando duas técnicas de congelação (convencional e automatizada) sobre a motilidade, vigor e resistência térmica dos espermatozóides após a descongelação. O experimento foi elaborado em um período de 12 semanas, sendo dividido em duas partes, uma inicial de seis semanas onde foi utilizado o diluidor Tris-gema e uma segunda parte na qual o diluidor utilizado foi Citrato-gema. Foram utilizados três touros holandeses para coleta de sêmen semanal pelo método da vagina artificial. Após a coleta, foram avaliados: volume, motilidade, turbilhonamento, vigor e concentração espermática para determinação do número de doses. Após diluição, o sêmen foi envasado em palhetas de 0,5 ml. Em seguida, as palhetas foram submetidas às técnicas de congelação convencional (uso de geladeira, vapor de nitrogênio e imersão em nitrogênio líquido) e automatizada (aparelho da Cryogen®). Após 24 horas as amostras foram descongeladas, reavaliadas e submetidas ao teste de termoresistência rápido (TTR). Os resultados foram analisados pelo teste Kruskal-Wallis (análise de variância não paramétrica) com nível de significância de 5% com auxílio dos programas BioEstat 4 e Systat 11. Houve diferença significativa (p<0,05) entre as técnicas automatizada e manual para os dois diluidores testados, sendo a técnica automatizada a que apresentou melhores resultados para motilidade e vigor após a criopreservação e teste de resistência térmica. Desta forma, pode-se concluir que a técnica automatizada para congelação de sêmen bovino apresenta-se vantajosa e prática pois além de proporcionar melhores resultados, ela padroniza as curvas de refrigeração e de congelamento.por
dc.description.abstractEven with technological advances in the field of artificial insemination, there is a huge number of people freezing semen in a manual way. This technique has proven to be viable, however, it is difficult to standardize the cooling and freezing curves, since they depend on the quality of the material used (refrigerator sealing, type of cooler, liquid nitrogen level and other). Therefore, the objective of this study was to evaluate the effect of the cryopreservation of bovine semen using two freezing techniques (conventional and automated) on motility, vigor and heat resistance of the sperm after thawing. The experiment was developed over a period of 12 weeks, divided into two parts, an initial six weeks part where Tris-yolk extender was used and a second part in which the extender used was Citrate-yolk. Three Holstein bulls were used for weekly semen collection by artificial vagina method. After collection, were evaluated: volume, motility, mass motility and sperm concentration to find out the number of doses. After dilution, the semen was stored in straws of 0.5 ml. Then the straws were subjected to conventional freezing technique (use of refrigerator, nitrogen vapor and immersion in liquid nitrogen) and automated technique (machine from Cryogen ®). After 24 hours the samples were thawed, re-tested and underwent the quick thermoresistance test. The results were analyzed by Kruskal-Wallis analysis of variance (nonparametric) with a significance level of 5% with the aid of BioEstat 4 and Systat 11 PC-programs. There was significant difference (p <0.05) between automated and manual techniques for both extenders used, and the automated technique has presented the best results for motility and vigor after cryopreservation and thermoresistance test. Thus, we can conclude that the automated technique to freezing bovine semen has shown to be advantageous and practical because as well as providing better results, it standardizes cooling and freezing curves.eng
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dc.languageporpor
dc.publisherUniversidade Federal Rural do Rio de Janeiropor
dc.publisher.departmentInstituto de Zootecniapor
dc.publisher.countryBrasilpor
dc.publisher.initialsUFRRJpor
dc.publisher.programPrograma de Pós-Graduação em Zootecniapor
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Reproduction in Domestic Animals, v.31, p. 135-140,1999. WATSON, P. F. The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, v. 60-1, p. 481-492, 2000. WOELDERS, H., MATHIIJS, A., ENGEL, B. Effects of trehalose, and sucrose, osmalality of the freezing medium, and cooling rate on viability and intractness of sperm after freezing and thawing. Cryobiology, v.35, p.93-105, 1997.por
dc.rightsAcesso Abertopor
dc.subjectcryopreservationeng
dc.subjectextendereng
dc.subjectcriopreservaçãopor
dc.subjectdiluidorespor
dc.subjectautomatizadopor
dc.subjectautomatedpor
dc.subject.cnpqCiências Agráriaspor
dc.titleEficiência da congelação automatizada na viabilidade de sêmen bovino.por
dc.title.alternativeEfficiency of automated freezing on the viability of bovine semen.eng
dc.typeDissertaçãopor
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