Contribuição ao estudo químico de Ouratea microdonta e Sauvagesia erecta (Ochnaceae), do látex de Parahancornia amapa (Apocynaceae) e atividade biológica

Abstract

The analysis of the fractions obtained from extracts of Ouratea microdonta (Ochnaceae) Sauvagesia erecta (Ochnaceae) and latex Parahancornia amapa (Apocynaceae) led to the isolation of constituents of different classes of metabolites special. O. microdonta (Ochnaceae) were isolated terpenes, sitosterol, stigmasterol, lupeol, α and β-amyrin acid, 3,5- dihydroxy-4-methoxybenzoic acid and biflavonoids, amenthoflavone (5,7,4'- trihydroxyflavone (3'-8'')-5'',7'',4'''-trihydroxyflavone), 7''-O-methyl-agathisflavone and agathisflavone (5,7,4'-trihydroxyflavone (6-8)-5'',7'',4'''-trihydroxyflavone). Of this metabolite derivative was obtained tetramethyl-agathisflafone through of data from the curves circulates dichroism (CD) and ultra violet (UV) could be established the absolute stereochemistry of biflavonoids these atropisomers. S. erecta was possible the isolation of terpenes, sitosterol and stigmasterol, ursolic acid, lupeol, α and β-amyrin, terpenes glycosylated 3-β-O-β-Dglucopyranosyl β-sitosterol and 3-O-β-D-glucopyranosyl stigmasterol, isoflavone 5,7,4'- trimetoxy-isoflavone, and flavonoid vitexin. P. amapa latex were isolated a mixture of pentacyclic triterpene lupeol, α-amyrin and β-amyrin and esters (3-O-acyl), predominantly esters of 3-O-acyl-lupeol, dienone the 1-hydroxy-4-oxo-2,5-dien-cyclohexyl-1-ethylene-Oglucopyranoside and sugar known as cornosideo metilmioinositol. The structures were identified by analysis of IR, Mass and 1H and 13C NMR, including 1D and 2D special techniques of natural substances and derivatives, and compared with data obtained from the literature. Tests were conducted to evaluate the potential of molluscicidal O. microdonta on Biomphalaria glabrata (mollusk), and the effects of latex P. amapa on the post embryonic development of the larvae of Chrysomya megacefhala and insecticide on larvae Chrysomya putoria, and the effect on ticks Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (tick). The metabolomic profile latex P. amapa confirmed the isolated and indicated that biological effects can be attributed to terpenes and dienone identified.