Plasma rico em plaquetas e mastite bovina: protocolo de obtenção, atividade in vitro e resposta da glândula mamária

Abstract

Aimed to standardize a manual technique for obtaining autologous platelet-rich plasma (PRP) in cattle for use in clinical practice. Blood (450 mL) from nine cows was divided into aliquots for test eight protocols (double centrifugation) which varied as the speed and time of centrifugation. All protocols (P1 to P8) were efficient to concentrate platelets being P5 and P8 the most concentrated platelets (890.53 ± 277.14 and 702.23 ± 298.8% compared to whole blood) (p>0.05). Comparing with the P5, P8 showed a higher number of leukocytes and obtained higher concentration of TGF-β1 (2422.67 ± 509.97 pg/mL) (p<0.05). Because of good results, protocols P5 and P8, were used to assess the antibacterial effect of pure PRP, platelet-poor plasma (PPP), PRP and PPP gel and plasma (P) against a standard strain of S. aureus. The highest antimicrobial activity was observed for PRP gels of both protocols (p> 0.05), confirming the importance of the second centrifugation with greater speed and time, and platelet activation to obtain better results. In vivo assays from intramammary application of PRP in cows with subclinical mastitis was observed systemic effect, has changed the red blood cells and was mobilizing blood leukocytes with consequent increase in somatic cells, but reduced oxidative stress in the gland suggesting positive actions of PRP as chemotactic agent and reduced deleterious activity in the tissues