Comparação entre duas técnicas coprológicas para o diagnóstico de habronemose gástrica dos eqüídeos

Abstract

The habromatids biology and their patogenies have abready been well studied. But not na efficient technique has been developed, or peridically tested for the diagnosis of the gastric habronemiasis in equidae. Has been proposed the compation of two techniques: the UENO (1986) technique and UENO & GUTIERRES (1983), modified and the xenodiagnostic technique. On the Ueno (modified) technique to recuperate first stage larvae, has been used fecal examples of 3g and 5g, with 3 repetitions for each. From each example has been taken 0,3 ml of the sediment, divided in three microscope slides and then the slides were observed though the light microscope for counting the larvae number of Habronema spp. or Drashia megastoma. To the xenodiagnostic, larvae of Musca domestica and Stomoxys calcitrans (Muscidae: Diptera) were inoculated in the equidae feces and then were kept in the laboratory. The adults muscoids0 were dissected and the third stage larvae of the nematodes were saved and counted. The adults nematodes were colected from the stomach of 35 equidae then fixed in AFA and preserved in alcohol 70% for later identification. The comparative analysis were based on the linear correlation. The comparation of the determination rates indicated that there weren’t significative difference on the recuperation of larvae on the Ueno (modified) technique, with the two different examples weights and the xenodiagnostic technique. The Ueno (modified) technique utilizing 3g and 5g of feces had the same index of determination, 73%. The test t analysis showed no significant difference between the three slides on the Ueno technique observation. While the xenodiagnostic technique got a determination rate of 79,1%, when utilizing only M. domestica and the two especies of females of Habronema spp. And the determination index of 81,3% when utilizing M. domestica and females of Habronema muscae based on the linear correlation.